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FEBS Lett. 2000 Apr 21;472(1):53-6.

Myocyte enhancer factor 2C upregulates MASH-1 expression and induces neurogenesis in P19 cells.

Author information

1
Department of Biochemistry, Medical Sciences Building, University of Western Ontario, London, Ont., Canada. skerjanc@julian.uwo.ca

Abstract

MEF2C is a transcription factor expressed in neural lineages. After transient transfection, the MEF2 family of factors can act synergistically with the neural-specific transcription factor, MASH-1, and activate exogenous neural-specific promoters. To determine whether MEF2C is capable of modulating endogenous gene expression, P19 cell lines were analyzed that overexpressed MEF2C, termed P19[MEF2C] cells. Here we show that P19[MEF2C] cells differentiate into neurons when aggregated with ME(2)SO. MEF2C-induced neurons expressed neurofilament protein, the nuclear antigen NeuN, as well as MASH-1. Our results indicate that MEF2C can directly or indirectly activate the expression of MASH-1, leading to neurogenesis.

PMID:
10781804
DOI:
10.1016/s0014-5793(00)01438-1
[Indexed for MEDLINE]
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