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FEBS Lett. 2000 Apr 21;472(1):50-2.

MAPK-dependent expression of p21(WAF) and p27(kip1) in PMA-induced differentiation of HL60 cells.

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1
Department of Pharmacology, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA.

Abstract

Treatment of HL60 cells with phorbol 12-myristate 13-acetate (PMA) results in growth arrest and differentiation towards the macrophage lineage. PMA-induced changes are easily monitored by morphological changes while cells in suspension start adhering onto substrate. PMA induces rapid activation of the extracellular signal-regulated kinases (ERKs). Activation of the ERK pathway is essential to PMA-induced differentiation of HL60 cells. PMA also induces the expression of the cyclin-dependent kinase inhibitors p21(WAF) and p27(kip1), which is modulated by the use of an inhibitor of the ERK cascade. This implies that a link exists between ERK activation and p21(WAF) and p27(kip1) induction in the process of terminal differentiation.

PMID:
10781803
DOI:
10.1016/s0014-5793(00)01416-2
[Indexed for MEDLINE]
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