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Hum Gene Ther. 2000 Apr 10;11(6):909-16.

Muscle transfection by electroporation with high-voltage and short-pulse currents provides high-level and long-lasting gene expression.

Author information

1
INSERM U 318, Laboratoire de Neurobiophysique-UJFG, Centre Hospitalier Universitaire, Grenoble, France. Jean-Michel.Vicat@ujf-grenoble.fr

Abstract

Gene transfer into muscle by electroporation with low-voltage and long-pulse (LV/LP, 100 V/50 msec) currents was shown to be more efficient than simple intramuscular DNA injection. Nevertheless, transgene expression declined from day 7 and only reached 10% of the maximum 3 weeks after electroporation. We have optimized electroporation conditions including voltage, pulse number, and the amount of injected luciferase-encoding plasmid DNA in the tibialis anterior muscle. Using high-voltage and short-pulse (HV/SP, 900 V/100 microsec) currents, we observed an average 500-fold increase in luciferase expression, in comparison with nonelectroporated muscle. Moreover, sustained and long-lasting gene expression was observed for at least 6 months. When we compared HV/SP currents with LV/LP currents, luciferase expression was similar 24 hr after electroporation. One month later, whereas luciferase expression was stable in muscle electroporated with HV/SP currents, it decreased 600-fold in muscle electroporated with LV/LP currents. In conclusion, electroporation with high-voltage and short-pulse currents provides high-level and long-lasting gene expression in muscle.

PMID:
10779167
DOI:
10.1089/10430340050015518
[Indexed for MEDLINE]

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