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Biophys J. 2000 May;78(5):2709-15.

Motion determination in actin filament fluorescence images with a spatio-temporal orientation analysis method.

Author information

1
Institut für Physiologie und Pathophysiologie, Ruprecht-Karls-Universität Heidelberg, 69120 Heidelberg, Germany. uttenweiler@urz.uni-heidelberg.de

Abstract

We present a novel approach of automatically measuring motion in series of microscopic fluorescence images. As a differential method, the three-dimensional structure tensor technique is used to calculate the displacement vector field for every image of the sequence, from which the velocities are subsequently derived. We have used this method for the analysis of the movement of single actin filaments in the in vitro motility assay, where fluorescently labeled actin filaments move over a myosin decorated surface. With its fast implementation and subpixel accuracy, this approach is, in general, very valuable for analyzing dynamic processes by image sequence analysis.

PMID:
10777767
PMCID:
PMC1300860
DOI:
10.1016/S0006-3495(00)76815-9
[Indexed for MEDLINE]
Free PMC Article

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