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Biophys J. 2000 May;78(5):2257-69.

Lateral diffusion of membrane proteins in the presence of static and dynamic corrals: suggestions for appropriate observables.

Author information

1
Department of Chemistry, University of California, San Diego, La Jolla, California 92093-0339 USA. flb@chem.ucsd.edu

Abstract

We consider the possibility of inferring the nature of cytoskeletal interaction with transmembrane proteins via optical experiments such as single-particle tracking (SPT) and near-field scanning optical microscopy (NSOM). In particular, we demonstrate that it may be possible to differentiate between static and dynamic barriers to diffusion by examining the time-dependent variance and higher moments of protein population inside cytoskeletal "corrals." Simulations modeling Band 3 diffusion on the surface of erythrocytes provide a concrete demonstration that these statistical tools might prove useful in the study of biological systems.

PMID:
10777724
PMCID:
PMC1300817
DOI:
10.1016/S0006-3495(00)76772-5
[Indexed for MEDLINE]
Free PMC Article

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