Format

Send to

Choose Destination
Biochem Biophys Res Commun. 2000 Apr 29;271(1):164-9.

Small heat shock protein 27 (HSP27) associates with tubulin/microtubules in HeLa cells.

Author information

1
Department of Biological Science, Faculty of Science, Hiroshima University, Higashi-Hiroshima, 739-8526, Japan.

Abstract

One of the monoclonal antibodies raised against mitotic HeLa cells (termed as mH3) recognized a 27-kDa protein and stained microtubules in the mitotic spindles of HeLa cells. Immunoscreening of a HeLa cDNA library revealed that mH3 antigen is a small heat shock protein, HSP27. Immunoprecipitation analysis using mH3 suggested that both alpha- and beta-tubulin are associated with HSP27. Further, sucrose-cushioned ultra centrifugation revealed that HSP27 is co-sedimented with taxol-stabilized microtubules. These results indicate that HSP27 associates with tubulin/microtubules in HeLa cells.

PMID:
10777697
DOI:
10.1006/bbrc.2000.2553
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center