[Use of recombinant protein E2 of the classical swine fever virus for immunization of animals]

V V Tsibezov; V S Bogdanova; A D Zaberezhny; T V Grebennikova; A V Krivonos; L A Dudnikov; S L Kal'nov; T I Aliper; E A Nepoklonov

[Use of recombinant protein E2 of the classical swine fever virus for immunization of animals]

Vopr Virusol. 2000 Mar-Apr;45(2):36-41.

[Article in Russian]

Authors

V V Tsibezov, V S Bogdanova, A D Zaberezhny, T V Grebennikova, A V Krivonos, L A Dudnikov, S L Kal'nov, T I Aliper, E A Nepoklonov

PMID: 10765549

Abstract

Recombinant major surface glycoprotein E2 from virulent Shimen strain of classical swine fever virus (CSFV) has been tested for immunogenicity in animal immunization experiments. Immunization of 3-month-old piglets with 200 micrograms of recombinant protein protected the animals from lethal challenge with virulent CSFV strain. CSFV-specific antibody detection test based on competitive ELISA has been developed using the recombinant E2 protein. The test can evaluate specific antibody levels after subunit vaccination with recombinant E2 after immunization with live vaccine based on attenuated CSFV strain.

Publication types

English Abstract

MeSH terms

Animals
Antibodies, Viral / blood
Chromatography, Affinity
Classical Swine Fever / prevention & control*
Enzyme-Linked Immunosorbent Assay
Recombinant Proteins / administration & dosage*
Recombinant Proteins / immunology
Recombinant Proteins / isolation & purification
Swine
Viral Envelope Proteins / administration & dosage*
Viral Envelope Proteins / immunology
Viral Envelope Proteins / isolation & purification
Viral Vaccines / administration & dosage*
Viral Vaccines / immunology

Substances

Antibodies, Viral
Recombinant Proteins
Viral Envelope Proteins
Viral Vaccines
glycoprotein E2, classical swine fever virus