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Arch Microbiol. 2000 Mar;173(3):229-31.

Construction and screening of Mycobacterium paratuberculosis insertional mutant libraries.

Author information

1
AgResearch, Wallaceville Animal Research Centre, Upper Hutt, New Zealand. cavaignacs@agresearch.cri.nz

Abstract

Mycobacterium paratuberculosis causes Johne's disease, a common wasting disease in ruminants. As a first step in studying virulence mechanisms, libraries of random mutants were produced in two M. paratuberculosis strains by using the conditionally replicating shuttle phasmid phAE94 which contains the transposon Tn5367. Two thousand mutants were screened for auxotrophy, carbon source preference, and altered cell wall. Genes interrupted by insertion were identified for seven mutants isolated from the screening process. Two mutants had insertions in putative genes involved in synthesis of the cell wall.

PMID:
10763756
DOI:
10.1007/s002039900132
[Indexed for MEDLINE]

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