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Am J Hum Genet. 2000 May;66(5):1684-8. Epub 2000 Apr 4.

An optimized probe set for the detection of small interchromosomal aberrations by use of 24-color FISH.

Author information

1
Institut für Anthropologie und Humangenetik, Ludwig-Maximilians-Universität München, Munich, Germany.

Abstract

The rapid spread of the use of new 24-color karyotyping techniques has preceded their standardization. This is best documented by the fact that the exact resolution limits have not yet been defined. Indeed, it is shown here that a substantial proportion of interchromosomal aberrations will be missed by all multicolor karyotyping systems currently in use. We demonstrate that both the sensitivity and the specificity of 24-color karyotyping critically depend on the fluorochrome composition of chromosomes involved in an interchromosomal rearrangement. As a solution, we introduce a conceptual change in probe labeling. Seven-fluorochrome sets that overcome many of the current limitations are described, and examples of their applications are shown. The criteria presented here for an optimized probe-set design and for the estimation of resolution limits should have important consequences for pre- and postnatal diagnostics and for research applications.

PMID:
10762552
PMCID:
PMC1378025
DOI:
10.1086/302875
[Indexed for MEDLINE]
Free PMC Article

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