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Parasitology. 2000 Mar;120 ( Pt 3):281-7.

Interferon-gamma induces secretion of trypanosome lymphocyte triggering factor via tyrosine protein kinases.

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Division for Infectious Diseases, Huddinge University Hospital, Sweden.


Bidirectional signalling between Trypanosoma brucei brucei and CD8+ T cells involves reciprocal action of the parasite-derived trypanosome derived lymphocyte triggering factor (TLTF) and lymphocyte-derived IFN-gamma. Herein we further characterize this relationship and report quantitation of TLTF secretion into culture supernatants using a newly developed ELISA. Secretion is induced by IFN-gamma in a dose-response manner, with 100 U/ml giving maximal yields of bioactive TLTF as assessed by ELISA and ELISPOT. This is a constitutive and active secretion. Furthermore, IFN-gamma-induced secretion was dependent on tyrosine protein kinase activity. Specific blockers of this signalling system resulted in lower yields of TLTF in the culture supernatants.

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