Send to

Choose Destination
Immunity. 2000 Mar;12(3):335-45.

Functional assessment of precursors from murine bone marrow suggests a sequence of early B lineage differentiation events.

Author information

Oklahoma Medical Research Foundation, Oklahoma City 73104, USA.


Most lineage marker-negative (Lin-)TdT+ cells from murine marrow lack CD34 but display c-kit at low density as well as IL-7Ralpha and Flk-2/Flt-3 receptors. Single cells with these characteristics generated CD45RA+CD19- as well as CD19+ lymphocytes in culture. CD45RA+CD19- marrow cells were resolved into three nonoverlapping subsets. One subset, lacking DX5 and Ly-6C antigens, yielded CD19+ cells in culture. Further analysis demonstrated CD24 on most Lin-TdT+ cells and all CD45R+CD19-DX5-Ly-6C- cells. Mac-1/CD11b was absent from these two subsets of B lineage precursors, while IL-7Ralpha was retained during subsequent differentiation to a CD19+ and stromal cell-independent stage. These findings contrast with previous descriptions of B lymphocyte precursors and suggest a sequence of early differentiation events.

[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center