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Nephron. 2000 Apr;84(4):362-6.

Protective effect of pyruvate upon cultured mesothelial cells exposed to 2 mM hydrogen peroxide.

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  • 1Department of Nephrology and Hypertension and Research Center for Experimental Nephrology, Ha' Emek Medical Center, Afula, Israel.


Rat peritoneal mesothelial cells in culture have the capability of generating hydrogen peroxide. Exposure of these cells to glucose-enriched, lactated-buffered fluids for peritoneal dialysis significantly increases the production of H(2)O(2). Increased liberation of oxygen radicals also involves the risk of damaging the peritoneal membrane. Pyruvate being a natural oxidant scavenger abundantly present in mammalian cells, we hypothesized that its protective effects facing H(2)O(2) can eventually be of relevance for the mesothelial monolayer of patients on long-term peritoneal dialysis. So far, we designed an experimental study in which rat peritoneal mesothelial cells in culture were exposed to 2 mM H(2)O(2). Cell damage was estimated in terms of decreased capability of the mitochondrial dehydrogenases to reduce MTT. Addition of 2 mM sodium pyruvate to the medium prevented the negative effect of hydrogen peroxide. The MTT/protein values for the control group were 0.00357 +/- 0.00075. The ratio after exposure to 2 mM H(2)O(2) was 0. 00217 +/- 0.00028, whereas that detected in cells incubated in H(2)O(2) plus pyruvate was 0.00325 +/- 0.0082 (p < 0.05). These results indicate that pyruvate protected rat peritoneal mesothelial cells in culture against oxidant injury. These data are one more piece of evidence pointing at pyruvate as a potentially useful buffer for peritoneal dialysis solutions.

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