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Planta. 2000 Feb;210(3):439-45.

The targeting and accumulation of ectopically expressed oleosin in non-seed tissues of Arabidopsis thaliana.

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1
IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Briston, Long Ashton, UK.

Abstract

Full-length and N-terminal deletions of a sunflower (Helianthus annuus L.) oleosin protein were expressed ectopically in transgenic Arabidopsis thaliana (L.) Heynh. Immunological detection of the sunflower protein revealed that it accumulated in a range of non-oil-storing tissues, including leaves, roots and petals. This accumulation was shown to result from deposition in the microsomal membrane fraction. Expression in oil-storing tissues (such as seeds) of oleosin N-terminal deletions revealed impaired transfer from the endoplasmic reticulum to the oil body. In non-oil-storing tissues, accumulation in the microsomal membrane fraction was progressively reduced by N-terminal deletion. These data confirm the role of the endomembrane system in the targeting of the oleosin and its intimate relationship with oil-body biogenesis.

PMID:
10750902
DOI:
10.1007/PL00008152
[Indexed for MEDLINE]
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