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Eur J Clin Microbiol Infect Dis. 2000 Feb;19(2):121-3.

Survival and growth of Burkholderia cepacia within the free-living amoeba Acanthamoeba polyphaga.

Author information

1
Department of Microbiology, University of Leeds, UK.

Abstract

The aim of this study was to investigate whether Burkholderia cepacia is capable of survival and growth within the free-living amoeba Acanthamoeba polyphaga using a differential immunofluorescence assay of bacterial-amoebal cocultures and viable counts of bacteria determined after amoebal lysis. The numbers of intra-amoebal bacteria and the numbers of infected amoebae increased over time; although, when heat-killed bacteria were used, no intracellular bacteria were observed. These findings should be taken into account in future studies of environmental reservoirs of Burkholderia cepacia.

PMID:
10746499
DOI:
10.1007/s100960050442
[Indexed for MEDLINE]

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