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Mol Gen Genet. 1979 Jan 5;168(1):111-5.

High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA.

Abstract

A highly efficient method for transformation of Bacillus subtilis by plasmid DNA is reported. The procedure, which involves polyethylene glycol-induced DNA uptake by protoplasts and subsequent regeneration of the bacterial cell wall, yields up to 80% transformants with an efficiency of 4 x 10(7) transformants per microgram of supercoiled DNA. Plasmids constructed by in vitro ligation or endonuclease-generated fragments of linear plasmid DNA can also transform PEG-treated protoplasts, but at a lower frequency.

PMID:
107388
DOI:
10.1007/bf00267940
[Indexed for MEDLINE]

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