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Vaccine. 2000 May 8;18(22):2426-34.

Immunogenicity of dengue virus type 1 DNA vaccines expressing truncated and full length envelope protein.

Author information

1
Virology Program, Infectious Diseases Department, Naval Medical Research Institute, 8901 Wisconsin Avenue, Bethesda, MD, USA. ravik@nmripo.nmri.nnmc.navy.mil

Abstract

Recombinant plasmid DNA constructs expressing truncated or full-length dengue-1 envelope (E) with or without the pre-membrane (prM) were tested for immunogenicity in mice, as candidate dengue DNA vaccines. Two plasmids, one expressing the N-terminal 80% E and the other expressing prM and full length E were immunogenic in intradermally inoculated mice. The vaccinated mice produced dengue-1 specific antibodies that were both neutralizing and long lasting. Data suggested that the plasmid expressing prM and full length E produced virus like particles in transfected cells, and is probably a better immunogen compared to that expressing 80% E.

PMID:
10738100
DOI:
10.1016/s0264-410x(99)00570-8
[Indexed for MEDLINE]

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