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FEBS Lett. 2000 Mar 24;470(2):102-6.

Sterol metabolism and ERG2 gene regulation in the yeast Saccharomyces cerevisiae.

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Université Louis Pasteur, INRA, 28, rue de Herrlisheim, P.O. Box 507, 68021, Colmar, France.


Certain exogenously-supplied sterols, like ergost-8-enol, are efficiently converted into ergosterol in yeast. We have taken advantage of this property to study the regulation of the Delta8-Delta7-sterol isomerase-encoding ERG2 gene in an ergosterol auxotrophic mutant devoid of squalene-synthase activity. Ergosterol starvation leads to an 8-16-fold increase in ERG2 gene expression. Such an increase was also observed in wild-type cells either grown anaerobically or treated with SR31747A a sterol isomerase inhibitor. Exogenously-supplied zymosterol is entirely transformed into ergosterol, which represses ERG2 transcription. By contrast, exogenously-supplied ergosterol has little or no effect on ERG2 transcription.

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