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Mem Inst Oswaldo Cruz. 2000 Mar-Apr;95(2):221-3.

A simple method for human peripheral blood monocyte isolation.

Author information

1
Departamento de Patologia, FCS, Universidade de Brasília, DF, Brasil.

Abstract

We describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy coat cells. The use of simple laboratory equipment and a relatively cheap reagent makes the described method a convenient approach to obtaining human monocytes.

PMID:
10733742
DOI:
10.1590/s0074-02762000000200014
[Indexed for MEDLINE]
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