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Prev Vet Med. 2000 Mar 29;44(1-2):1-7.

Bulk-tank milk ELISA antibodies for estimating the prevalence of paratuberculosis in Danish dairy herds.

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Cattle Health Laboratory, Danish Dairy Board, Ladelundvej 85A, 6650, Brorup, Denmark.


Paratuberculosis (Johne's disease) has been widespread in Danish dairy herds for a long time but the herd-level prevalence has never been determined precisely. To evaluate the prevalence of paratuberculosis in Danish dairy herds in various regions, an ELISA based on a commercially available antigen was adapted for testing bulk-tank milk for the presence of antibodies to Mycobacterium avium subsp. paratuberculosis. Bulk-tank milk samples were collected from six milk-collecting centres from six different areas of the country. Samples from 900 herds (about 7.5% of all Danish dairy herds) were examined, and 70% were positive at the statistically optimal cut-off (sensitivity 97.1%; specificity 83.3%). The technical performance of the ELISA was not sufficient to provide a tool for surveillance because even slight changes in optical density for the samples would change the classification of some samples. The infection is more widespread than previous investigations have shown.

[Indexed for MEDLINE]

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