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Electrophoresis. 2000 Feb;21(3):645-53.

Protein patterns of gel-entrapped Escherichia coli cells differ from those of free-floating organisms.

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1
UMR 6522 CNRS, Faculté des Sciences de Rouen, Mont-Saint-Aignan, France.

Abstract

The two-dimensional electrophoretic patterns of cellular proteins from gel-entrapped Escherichia coli cells were compared to those of exponential- and stationary-phase free-floating organisms. The amounts of several proteins in immobilized cells were significantly different from those in free bacteria. Immobilized organisms rapidly produced a high level of dipeptide permease and a single-strand binding protein, and progressively accumulated an aldehyde dehydrogenase. Immobilization also induced a decrease in the levels of two proteins, i.e., the YFID protein and a DNA-binding, stationary-phase protein. The possible role of these proteins in the high resistance of immobilized bacteria to stresses is discussed.

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