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Virus Res. 2000 Feb;66(2):187-96.

Cell surface expression of immature H glycoprotein in measles virus-infected cells.

Author information

1
Department of Virology, Osaka City University Medical School, Asahimachi 1-4-3, Abeno-ku, Osaka, Japan. ogurah@med.osaka-cu.ac.jp

Abstract

Two forms of hemagglutinin (H) protein, one with an apparent molecular mass of 78 kDa (78K H protein) and the other with that of 74 kDa (74K H protein), are present in cells infected with measles virus (MV). We previously observed that only the mature 78K H protein, a completely glycosylated form of the 74K H protein, was expressed on the cell surface of the infected cells. In the present study, we detected transient expression of the 74K H protein on the cell surface of infected cells by pulse-chase studies, although the level of this expression was much lower than that of the 78K H protein. On the cell surface the 74K H protein was present as dimers and sensitive to endo-beta-N-acetylglucosaminidase H digestion. Treatment with brefeldin A, which blocks the transport of membrane and secretory proteins from the endoplasmic reticulum to the Golgi apparatus, inhibited the cell surface expression of the 78K H protein, but not that of the 74K H protein. These data suggest that a part of the MV 74K H proteins could be transported directly to the cell surface - probably via an alternative pathway - without processing to the complex form in the Golgi apparatus.

PMID:
10725551
DOI:
10.1016/s0168-1702(00)00124-6
[Indexed for MEDLINE]

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