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J Gen Virol. 2000 Apr;81(Pt 4):1033-40.

Glycoprotein G of herpes simplex virus type 1: identification of type-specific epitopes by human antibodies.

Author information

1
Departments of Clinical Virology and Dermatovenereology, Göteborg University, Guldhedsgatan 10 B, S-413 46, Göteborg, Sweden.

Abstract

Serological diagnosis of herpes simplex virus (HSV) infections requires assays based on antigens that expose type-specific determinants. This study was designed to outline the B-cell epitopes of the type-specific glycoprotein G-1 (gG-1) of HSV type 1 (HSV-1), by investigating the reactivity of human anti-gG-1 antibodies, purified from 21 HSV-1-isolation-proven patient sera, to cellulose-bound synthetic peptides spanning the entire gG-1 sequence. The epitope mapping demonstrated that these antibodies bound preferentially to antigenic determinants that localized to regions with a high degree of amino acid similarity to the corresponding glycoprotein in HSV-2, gG-2. In spite of this, the purified anti-gG-1 antibodies were found to be non-reactive to native gG-2 antigen, as well as to overlapping gG-2 peptides, thus supporting the role of gG-1 as a prototype HSV-1 type-specific antigen. One immunodominant region, delimited by amino acids 112-127, reacted with all purified anti-gG-1 antibodies and may be of interest for the further development of a peptide-based HSV-1 type-specific seroassay.

PMID:
10725430
DOI:
10.1099/0022-1317-81-4-1033
[Indexed for MEDLINE]

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