Isolation and characterization of a novel TP53-inducible gene, TP53TG5, which suppresses growth and shows cell cycle-dependent transition of expression

S Isaka; Y Takei; T Tokino; K Koyama; Y Miyoshi; M Suzuki; E Takahashi; C Azuma; Y Murata; Y Nakamura

doi:10.1002/(sici)1098-2264(200004)27:4<345::aid-gcc2>3.0.co;2-3

Isolation and characterization of a novel TP53-inducible gene, TP53TG5, which suppresses growth and shows cell cycle-dependent transition of expression

Genes Chromosomes Cancer. 2000 Apr;27(4):345-52. doi: 10.1002/(sici)1098-2264(200004)27:4<345::aid-gcc2>3.0.co;2-3.

Authors

S Isaka¹, Y Takei, T Tokino, K Koyama, Y Miyoshi, M Suzuki, E Takahashi, C Azuma, Y Murata, Y Nakamura

Affiliation

¹ Division of Clinical Genetics, Department of Medical Genetics, Biomedical Research Center, Osaka University Medical School, Osaka, Japan.

PMID: 10719363
DOI: 10.1002/(sici)1098-2264(200004)27:4<345::aid-gcc2>3.0.co;2-3

Abstract

Through a strategy of direct cloning of TP53-binding DNA sequences from human genome DNA, we have identified a novel TP53-target gene, termed TP53TG5 (TP53-target gene 5). This gene, localized to chromosome band 20q13.1 by fluorescence in situ hybridization, encodes a 290-amino-acid peptide with no significant homology with any known proteins in the public database. A colony-formation assay using human glioblastoma cell line T98G, which lacks wild-type TP53 and expresses no endogenous TP53TG5, revealed a growth-suppressive effect of the TP53TG5 gene product. Furthermore, immunohistochemical studies, following transfection of T98G with plasmid designed to express green fluorescent protein-fused TP53TG5, revealed cell cycle-dependent intracellular localization of this protein. Our results suggest that functional studies of TP53TG5 may provide new insights into the complex physiological activities of TP53.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adaptor Proteins, Signal Transducing
Amino Acid Sequence
Antineoplastic Agents / pharmacology
Cell Cycle Proteins / chemistry
Cell Cycle Proteins / genetics*
Cell Cycle Proteins / isolation & purification
Cell Cycle Proteins / physiology
Chromosomes, Human, Pair 20 / genetics
Cloning, Molecular
DNA-Binding Proteins / chemistry*
DNA-Binding Proteins / genetics*
DNA-Binding Proteins / isolation & purification
DNA-Binding Proteins / physiology
Female
Gene Expression Regulation / physiology*
Genes, p53*
Growth Inhibitors / physiology*
Humans
Male
Microscopy, Fluorescence
Molecular Sequence Data
Tumor Cells, Cultured

Substances

Adaptor Proteins, Signal Transducing
Antineoplastic Agents
Cell Cycle Proteins
DNA-Binding Proteins
Growth Inhibitors
TP53TG5 protein, human

Associated data

GENBANK/AB017802