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FEMS Microbiol Lett. 2000 Mar 15;184(2):231-5.

Evidence for the transport of zinc(II) ions via the pit inorganic phosphate transport system in Escherichia coli.

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Division of Life Sciences, King's College London, Campden Hill Road, London, UK.


A locus involved in zinc(II) uptake in Escherichia coli K-12 was identified through the generation of a zinc(II)-resistant mutant by transposon (Tn10dCam) mutagenesis. The mutation was located within the pitA gene, which encodes the low-affinity inorganic phosphate transport system (Pit). The pitA mutant accumulated reduced amounts of zinc(II) when exposed to 0.5-2.0 mM ZnSO(4) during growth in Luria-Bertani medium.

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