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FEMS Microbiol Lett. 2000 Mar 15;184(2):225-9.

Identification of iron-regulated genes of Helicobacter pylori by a modified fur titration assay (FURTA-Hp).

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1
University of Freiburg, Institute of Medical Microbiology and Hygiene, Department of Microbiology, Hermann-Herder-Str. 11, D-79104, Freiburg, Germany.

Abstract

The Escherichia coli-based Fur titration assay (FURTA), although a powerful tool for identification of genes regulated by the ferric uptake regulator (Fur), was unsuccessful for the gastric pathogen Helicobacter pylori. The FURTA was modified by construction of an E. coli indicator strain producing H. pylori Fur only. The promoter regions of the ferric citrate receptor homolog fecA2 and the riboflavin synthesis gene ribBA were both positive in the modified FURTA, but negative in the original FURTA. Transcription of fecA2 and ribBA was demonstrated to be iron-repressed in H. pylori. This type of modification should allow FURTA analysis for bacteria with Fur binding sequences poorly recognized by E. coli Fur.

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