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Nucl Med Biol. 1999 Nov;26(8):923-9.

High yield direct 76Br-bromination of monoclonal antibodies using chloramine-T.

Author information

1
Department of Oncology, Radiology and Clinical Immunology, Uppsala University, Sweden. Johanna.Sundin@pet.uu.se

Abstract

Monoclonal antibody (MAb) A33 was labeled with the positron emitter 76Br (T(1/2) = 16.2 h). Direct labeling was done using the conventional chloramine-T method. After optimization of the labeling conditions, a maximum yield (mean +/- max error) of 77 +/- 2% was obtained at pH 6.8. In vitro binding of 76Br-A33 to SW1222 colonic cancer cells showed that the immunoreactivity was retained. Also, the MAbs 38S1 and 3S193 and the peptide hEGF were 76Br-labeled, resulting in labeling yields (mean +/- max error) of 75 +/- 3%, 63 +/- 4%, and 73 +/- 0.1%, respectively. We conclude that antibodies and peptides can be labeled conveniently with 76Br for the purpose of whole-body tumour imaging by positron emission tomography.

PMID:
10708306
DOI:
10.1016/s0969-8051(99)00076-1
[Indexed for MEDLINE]

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