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Anal Chem. 2000 Feb 15;72(4):827-31.

Separation of nontarget compounds by DNA aptamers.

Author information

1
Department of Chemistry, P. M. Gross Chemical Laboratory, Duke University, Durham, North Carolina 27708-0346, USA.

Abstract

The ability of DNA aptamers to separate nontarget compounds is demonstrated. Two G-quarter forming aptamers, a 15-mer and a 20-mer, were covalently linked to fused silica capillary columns to serve as stationary-phase reagents in capillary electrochromatography. Separations of binary mixtures of amino acids (D-trp and D-tyr), enantiomers (D-trp and L-trp), and polycyclic aromatic hydrocarbons were achieved. Aptamers offer several attractive features for stationary-phase reagents, including ease of synthesis and of attachment to surfaces and modification of their binding properties through minor changes in sequence.

PMID:
10701269
DOI:
10.1021/ac991112f
[Indexed for MEDLINE]

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