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Mol Biochem Parasitol. 2000 Mar 5;106(2):261-71.

Cloning of the immunodominant 17-kDa antigen from Cryptosporidium parvum.

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Division of Parasitic Diseases, Centers for Disease Control and Prevention, Public Health Service, US Department of Health and Human Services, Mail Stop F-13, Building 23, Room 1025, 4770 Buford Highway N.E., Atlanta, GA, USA.


Infection with Cryptosporidium parvum causes a self-limiting diarrheal illness in immunocompetent humans and is associated with the development of a serum IgG antibody response dominated by the 27-kDa and 17-kDa parasite surface antigens. Antibodies against the 27-kDa and 17-kDa antigens may serve as useful markers for past infection in population-based studies of the risk factors associated with Cryptosporidium infection. A recombinant form of the 17-kDa antigen would be useful both in epidemiologic studies and in studies of the role of the humoral response in immunity. We have partially purified and sequenced the immunodominant 17-kDa surface antigen from sporozoites, and we have cloned a 975 bp open reading frame from C. parvum that includes all of the 17-kDa antigen peptide sequences. We show immunologic identity between a recombinant form of the protein and the native 17-kDa antigen. We conclude that the carboxy-terminal fragment of the cloned protein is the authentic 17-kDa antigen.

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