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Science. 2000 Mar 3;287(5458):1641-4.

A potassium channel protein encoded by chlorella virus PBCV-1.

Author information

1
Albrecht-von-Haller-Institut für Pflanzenwissenschaften, Universität Göttingen, 37073 Göttingen, Germany.

Abstract

The large chlorella virus PBCV-1, which contains double-stranded DNA (dsDNA), encodes a 94-codon open reading frame (ORF) that contains a motif resembling the signature sequence of the pore domain of potassium channel proteins. Phylogenetic analyses of the encoded protein, Kcv, indicate a previously unidentified type of potassium channel. The messenger RNA encoded by the ORF leads to functional expression of a potassium-selective conductance in Xenopus laevis oocytes. The channel blockers amantadine and barium, but not cesium, inhibit this conductance, in addition to virus plaque formation. Thus, PBCV-1 encodes the first known viral protein that functions as a potassium-selective channel and is essential in the virus life cycle.

PMID:
10698737
[Indexed for MEDLINE]
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