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Immunology. 2000 Feb;99(2):266-71.

Human B-cell lines constitutively express and secrete interleukin-16.

Author information

1
Laboratory of Cytokine Research, Department of Biology, University of West Florida, Pensacola, FL 32514, USA.

Abstract

Interleukin-16 (IL-16), produced by activated CD8+ T lymphocytes, is inhibitory to human immunodeficiency virus-1 (HIV-1) replication. In an attempt to determine whether human B cells express and secrete IL-16, a wide panel of B-cell lines derived from patients with acquired immune deficiency syndrome (AIDS)-associated B-cell lymphomas (AABCL) (n = 5) and from non-AABCLs (n = 8) were studied. Using reverse transcription-polymerase chain reaction (RT-PCR) analysis, we were able to observe ubiquitous expression of IL-16 mRNA. Kinetic studies on constitutive mRNA turnover and secretion for IL-16 suggests that the optimum expression is at 24 hr. Interestingly, we report, for the first time, IL-16 secretion by human B-cell lines.

PMID:
10692046
PMCID:
PMC2327161
DOI:
10.1046/j.1365-2567.2000.00959.x
[Indexed for MEDLINE]
Free PMC Article

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