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Neurosci Lett. 2000 Mar 3;281(1):61-4.

Systemic lipopolysaccharide and interleukin-1beta activate the interleukin 6: STAT intracellular signaling pathway in neurons of mouse trigeminal ganglion.

Author information

1
Neural Plasticity Research Group, Massachusetts General Hospital East, Building 149, 13th Street, CNY 4, Charlestown, MA 02129, USA. kobierski@helix.mgh.harvard.edu

Abstract

We have studied the activation of signal transducers and activators of transcription (STATs) in trigeminal ganglion after intraperitoneal injection of lipopolysaccharide (LPS) and Interleukin-1beta (IL-1beta). Using electrophoretic mobility shift assays we have shown that STAT1 and STAT3 are activated within 1 to 2 h. of injection of either LPS or IL-1beta. Eight hours after LPS injection the DNA binding activity of these complexes is still elevated while induction by IL-1beta returns to baseline levels within 4 h. By immunohistochemistry, using an antibody specific for the tyrosine phosphorylated, activated form of STAT-1, we show that this induction occurs in sensory neurons. IL-6 may be important in this cascade since induction of STATs by IL-1beta is blocked in Interleukin-6 knock out mice.

PMID:
10686416
DOI:
10.1016/s0304-3940(99)00953-2
[Indexed for MEDLINE]

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