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Cancer Lett. 2000 Jan 1;148(1):65-71.

Caspase-9 regulates cisplatin-induced apoptosis in human head and neck squamous cell carcinoma cells.

Author information

1
Department of Otorhinolaryngology, St. Marianna University School of Medicine, Kawasaki, Kanagawa, Japan.

Abstract

The aim of this study was to understand the molecular requirements for cisplatin-induced apoptosis in human head and neck squamous cell carcinoma (HNSCC) cell death. Cisplatin induced apoptosis in HNSCC cell lines, HSC-2, HSC-3 and HSC-4 in a dose-dependent manner. However, cisplatin did not induce the expression of Fas-ligand mRNA or upregulation of Fas protein. By caspase activation assays, cisplatin induced Caspase-3 (Casp-3), -8 and -9 activation. In all three lines tested, the use of a specific inhibitor of Casp-9 almost completely blocked cisplatin-induced apoptosis, while the use of Casp-3 and -8 inhibitors resulted in a partial blockade of cisplatin-induced apoptosis. Our results strongly suggest that Casp-9-dependent apoptosis plays an important role in cisplatin-induced HNSCC apoptosis.

PMID:
10680594
DOI:
10.1016/s0304-3835(99)00315-8
[Indexed for MEDLINE]

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