Format

Send to

Choose Destination
Curr Opin Microbiol. 2000 Feb;3(1):97-101.

Novel approaches to monitor bacterial gene expression in infected tissue and host.

Author information

1
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111, USA.

Abstract

Elucidating the complex and dynamic host-microbe interactions during infection requires, among other things, detailed knowledge of microbial gene expression in vivo. Recently, advances in fluorescence and bioluminescence detection techniques, as well as recombinase-based in vivo expression technology, have rendered monitoring virulence gene expression in vivo a feasible task. These techniques have been adapted by several laboratories to study the spatial and temporal patterns of virulence gene expression by organisms such as Salmonella typhimurium, Listeria monocytogenes, Yersinia entercolitica and Vibrio cholerae during infection of tissue culture or animal models of infection.

PMID:
10679414
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center