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Mol Cell. 2000 Jan;5(1):85-95.

CDK inactivation is the only essential function of the APC/C and the mitotic exit network proteins for origin resetting during mitosis.

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ICRF Clare Hall Laboratories, South Mimms, Hertfordshire, United Kingdom.


Passage through mitosis is required to reset replication origins for the subsequent S phase. During mitosis, a series of biochemical reactions involving cyclin-dependent kinases (CDKs), the anaphase promoting complex or cyclosome (APC/C), and a mitotic exit network including Cdc5, 14, and 15 coordinates the proper separation and segregation of sister chromatids. Here we show that cyclin B/CDK inactivation can drive origin resetting in either early S phase or mitosis. This origin resetting occurs efficiently in the absence of APC/C function and mitotic exit network function. We conclude that CDK inactivation is the single essential event in mitosis required to allow pre-RC assembly for the next cell cycle.

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