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FEBS Lett. 2000 Feb 11;467(2-3):279-84.

Regulated but not constitutive human respiratory syncytial virus (HRSV) P protein phosphorylation is essential for oligomerization.

Author information

1
Centro Nacional de Microbiologia (C.N.M), Instituto de Salud Carlos III (ISCIII), Carretera Majadahonda-Pozuelo Km 2, Majadahonda, Madrid, Spain.

Abstract

Purified human respiratory syncytial virus (HRSV) P phosphoprotein from transfected HEp-2 cells is able to oligomerize forming tetramers. The bulk of constitutive P protein phosphorylation (99. 8%) (serine residues 116, 117, 119, 232 and 237) can be removed without affecting protein oligomerization. However, dephosphorylated P protein, produced in bacteria, is unable to oligomerize. This difference can be explained by a transient P protein phosphorylation, detected in HEp-2 cells, that could be essential for P protein oligomerization.

PMID:
10675554
DOI:
10.1016/s0014-5793(00)01171-6
[Indexed for MEDLINE]
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