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J Allergy Clin Immunol. 2000 Feb;105(2 Pt 1):327-32.

IL-4 production by PBMCs on stimulation with mite allergen is correlated with the level of serum IgE antibody against mite in children with bronchial asthma.

Author information

1
Department of Allergy and Clinical Immunology, Shizuoka Children's Hospital, Shizuoka, Japan.

Abstract

BACKGROUND:

Although IL-4, IL-13, and IFN-gamma are known to affect IgE synthesis, it remains unclear which one plays the most important role in in vivo IgE synthesis in atopic patients.

OBJECTIVE:

The aim of this study is to clarify the difference in importance among these cytokines in up-regulation of IgE synthesis in atopic patients.

METHODS:

We measured IL-4, IL-13, and IFN-gamma production by PBMCs on stimulation with house dust mite (HDM) in 23 children, 3 to 15 years old, with bronchial asthma (BA) and analyzed the correlation with HDM-specific IgE antibody levels expressed as HDM IgE radioallergosorbent test (RAST) results.

RESULTS:

The production of IL-4 and IL-13 by PBMCs on stimulation with HDM was significantly higher in children with BA than in nonatopic control subjects (IL-4, 752.9 +/- 365.9 vs 312.3 +/- 230.0 fg/mL, P <.001; IL-13, 21.9 pg/ml [<12.0-77.6] vs <12.0, P <.01). IL-4 production showed a close positive correlation with HDM IgE RAST (r = 0.71, P <.001), which was distinctly stronger than that between IL-13 production and HDM IgE RAST (r = 0.46, P <.05). IFN-gamma production was neither different between children with BA and nonatopic control subjects (7. 24 [1.54-33.90] pg/mL vs 11.2 [1.66-75.9] pg/mL) nor correlated with HDM IgE RAST levels. Essentially the same result was obtained by stimulation of PBMCs with a purified HDM major allergen Der f 1.

CONCLUSION:

IL-4 is likely to be the most important cytokine in up-regulation of in vivo IgE synthesis against HDM in children with BA.

PMID:
10669854
DOI:
10.1016/s0091-6749(00)90083-6
[Indexed for MEDLINE]

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