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Lett Appl Microbiol. 1999 Dec;29(6):406-10.

Development of a m-PCR assay for simultaneous identification of Campylobacter jejuni and C. coli.

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1
Unité d'Hygiène et Qualité des Produits Avicoles et Porcins, Agence Française de Sécurité Sanitaire des Aliments, Ploufragan, France.

Abstract

Multiplex PCR assay (m-PCR) with three sets of primers was developed for simultaneous identification of Campylobacter jejuni and C. coli. Poultry faecal samples were enriched in Preston broth for 24 h and streaking on selective media was performed before and after enrichment. m-PCR was applied on bacterial cultures harvested from media plates. The data showed a selective effect of Preston broth which favoured the growth of C. coli. Identification of the species by the hippurate hydrolysis test and by the m-PCR was performed on 294 isolates of Campylobacter. The efficiency of the identification by the biochemical test is only 34% in comparison to 100% efficiency with the PCR. The use of our m-PCR in combination with the culture method allowed reliable detection and identification of C. jejuni and C. coli within 3-4 d.

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