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Mol Plant Microbe Interact. 2000 Feb;13(2):232-7.

Small, stable shuttle vectors based on the minimal pVS1 replicon for use in gram-negative, plant-associated bacteria.

Author information

1
Laboratoire de Biologie Microbienne, Université de Lausanne, Switzerland.

Abstract

The minimal replicon of the Pseudomonas plasmid pVS1 was genetically defined and combined with the Escherichia coli p15A replicon, to provide a series of new, oligocopy cloning vectors (5.3 to 8.3 kb). Recombinant plasmids derived from these vectors were stable in growing and nongrowing cells of root-colonizing P. fluorescens strains incubated under different environmental conditions for more than 1 month.

PMID:
10659714
DOI:
10.1094/MPMI.2000.13.2.232
[Indexed for MEDLINE]
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