Format

Send to

Choose Destination
See comment in PubMed Commons below
Nat Biotechnol. 2000 Feb;18(2):208-12.

Identification and expression of isoflavone synthase, the key enzyme for biosynthesis of isoflavones in legumes.

Author information

1
Agricultural Biotechnology, The Dupont Company, Experimental Station; Wilmington, DE 19880-0402, USA.

Erratum in

  • Nat Biotechnol 2000 May;18(5):559.

Abstract

Isoflavones have drawn much attention because of their benefits to human health. These compounds, which are produced almost exclusively in legumes, have natural roles in plant defense and root nodulation. Isoflavone synthase catalyzes the first committed step of isoflavone biosynthesis, a branch of the phenylpropanoid pathway. To identify the gene encoding this enzyme, we used a yeast expression assay to screen soybean ESTs encoding cytochrome P450 proteins. We identified two soybean genes encoding isoflavone synthase, and used them to isolate homologous genes from other leguminous species including red clover, white clover, hairy vetch, mung bean, alfalfa, lentil, snow pea, and lupine, as well as from the nonleguminous sugarbeet. We expressed soybean isoflavone synthase in Arabidopsis thaliana, which led to production of the isoflavone genistein in this nonlegume plant. Identification of the isoflavone synthase gene should allow manipulation of the phenylpropanoid pathway for agronomic and nutritional purposes.

PMID:
10657130
DOI:
10.1038/72671
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group
    Loading ...
    Support Center