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J Clin Pathol. 1999 Sep;52(9):693-4.

Modified Genta triple stain for identifying Helicobacter pylori.

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1
Gastrointestinal Mucosa Pathology Laboratory, Veterans Affairs Medical Center, Houston, Texas, USA.

Abstract

AIM:

To evaluate whether lead nitrate could replace uranyl nitrate in the Genta stain for H pylori without sacrificing the advantages of the triple stain (Steiner silver impregnation combined with Alcian blue and haematoxylin/eosin (H&E)).

METHODS:

A comparison was made in 16 specimens between the original triple stain and the revised version. One pathologist evaluated all sections.

RESULTS:

Direct substitution of lead nitrate for uranium nitrate produced well stained organisms without interfering with H&E or Alcian blue staining. No difference was found in the ability to identify bacteria in 11 cases with H pylori density of 1 or 2 (on a scale of 0 to 5).

CONCLUSIONS:

The potential chemical and radiological hazards associated with uranium nitrate can be eliminated by using lead nitrate without sacrificing the advantages obtained by using the triple stain.

PMID:
10655993
PMCID:
PMC501547
[Indexed for MEDLINE]
Free PMC Article
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