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Nat Cell Biol. 2000 Feb;2(2):90-5.

DNA-replication/DNA-damage-dependent centrosome inactivation in Drosophila embryos.

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1
Department of Radiobiology, Faculty of Medicine, University of Groningen, Groningen, The Netherlands.

Abstract

During early embryogenesis of Drosophila melanogaster, mutations in the DNA-replication checkpoint lead to chromosome-segregation failures. Here we show that these segregation failures are associated with the assembly of an anastral microtubule spindle, a mitosis-specific loss of centrosome function, and dissociation of several components of the gamma-tubulin ring complex from a core centrosomal structure. The DNA-replication inhibitor aphidicolin and DNA-damaging agents trigger identical mitotic defects in wild-type embryos, indicating that centrosome inactivation is a checkpoint-independent and mitosis-specific response to damaged or incompletely replicated DNA. We propose that centrosome inactivation is part of a damage-control system that blocks chromosome segregation when replication/damage checkpoint control fails.

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PMID:
10655588
DOI:
10.1038/35000041
[Indexed for MEDLINE]

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