Format

Send to

Choose Destination
Eur J Neurosci. 2000 Jan;12(1):400-4.

Altering the concentration of GABA in the synaptic cleft potentiates miniature IPSCs in rat occipital cortex.

Author information

1
Institut Alfred Fessard, CNRS UPR 2212, 1, Avenue de la Terrasse, 91198 Gif-sur-Yvette, France.

Abstract

We have tested the effect of dextran (40 kDa, 5%) on miniature IPSCs (mIPSCs) recorded in layer V cortical pyramidal cells. This compound increases the amplitude of mIPSCs at room and physiological temperatures by 15%, leaving their duration unaffected at room temperature and slightly increased at physiological temperature. The amplitude increase is attributable to an increase in the number of receptors bound by GABA during synaptic transmission, as shown by the occlusion between the effects of dextran and zolpidem on mIPSC amplitude at room temperature. As dextran presumably enhances the concentration and dwell time of GABA in the synaptic cleft, these results demonstrate that the postsynaptic GABAA receptors are not saturated at room and physiological temperatures.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center