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Arch Toxicol. 1999 Nov;73(8-9):427-30.

Real-time PCR-analysis of the cytochrome P450 1B1 codon 432-polymorphism.

Author information

1
Institut für Arbeitsphysiologie an der Universität Dortmund, Germany.

Abstract

The aim of the present study was to develop and validate a rapid assay for genotyping of CYP1B1 codon 432-polymorphism. The described method is a single tube assay and combines both rapid-cycle polymerase chain reaction (PCR) with real-time monitoring by amplification and generation of the melting profiles of an allele-specific fluorescent probe. With this method 300 samples were analysed from healthy, unrelated Germans. Genotype frequency determined for the mutated allele CYP1B1*2 was 0.40. The results show that genotyping of CYP1B1 codon 432-polymorphism with a real-time fluorescence PCR method is a rapid and reliable assay for the analysis of large numbers of samples.

PMID:
10650913
DOI:
10.1007/s002040050631
[Indexed for MEDLINE]

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