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Nat Neurosci. 2000 Feb;3(2):120-5.

Identification and characterization of the high-affinity choline transporter.

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Department of Neurochemistry, Faculty of Medicine, University of Tokyo and CREST of Japan Science and Technology Corporation, Bunkyo-ku, Tokyo 113-0033, Japan.


In cholinergic neurons, high-affinity choline uptake in presynaptic terminals is the rate-limiting step in acetylcholine synthesis. Using information provided by the Caenorhabditis elegans Genome Project, we cloned a cDNA encoding the high-affinity choline transporter from C. elegans (cho-1). We subsequently used this clone to isolate the corresponding cDNA from rat (CHT1). CHT1 is not homologous to neurotransmitter transporters, but is homologous to members of the Na+-dependent glucose transporter family. Expression of CHT1 mRNA is restricted to cholinergic neurons. The characteristics of CHT1-mediated choline uptake essentially match those of high-affinity choline uptake in rat brain synaptosomes.

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