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FEBS Lett. 2000 Jan 21;466(1):29-34.

Inositol 1,4,5-trisphosphate receptor associated with focal contact cytoskeletal proteins.

Author information

1
Vessel Research Laboratory Co., Ltd., 3-6-6 Asahimachi, Machida-shi, Tokyo, Japan. sugiyama@hri.co.jp

Abstract

The linkage between inositol 1,4,5-trisphosphate (IP(3)) receptors (IP(3)Rs) and cytoskeletal proteins is considered to be important in cell function. In the present study, the association of IP(3)R subtypes with cytoskeletal proteins was examined using monoclonal antibodies specific to each IP(3)R subtype. We found that IP(3)R type 2 colocalized with talin, a focal contact cytoskeletal protein. IP(3)R type 2 exhibited a patchy distribution in the peripheral cytoplasm differently from type 1 and type 3 IP(3)R. Furthermore, IP(3)R subtypes co-immunoprecipitated with talin, vinculin and alpha-actin, but not alpha-actinin or paxillin.

PMID:
10648806
DOI:
10.1016/s0014-5793(99)01732-9
[Indexed for MEDLINE]
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