Format

Send to

Choose Destination
Nucleic Acids Res. 2000 Feb 15;28(4):E12.

A simplified method of generating transgenic Xenopus.

Author information

1
Division of Developmental Biology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK. dsparro@nimr.mrc.ac.uk

Abstract

Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of normally developing transgenic embryos.

PMID:
10648800
PMCID:
PMC102591
DOI:
10.1093/nar/28.4.e12
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center