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Laryngoscope. 2000 Jan;110(1):161-7.

Expression of angiogenic growth factors in paragangliomas.

Author information

1
Department of Otorhinolaryngology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA. rjyung@flower.aud.temple.edu

Abstract

OBJECTIVE/HYPOTHESIS:

To determine if angiogenic growth factors including vascular endothelial growth factor (VEGF) and platelet-derived endothelial cell growth factor (PD-ECGF) are expressed in human paragangliomas.

STUDY DESIGN:

A histopathologic and molecular examination of paraganglioma specimens obtained from surgical cases or retrieved from the Pathology Department of the Massachusetts Eye and Ear Infirmary.

METHODS:

Fresh tumor or archival, paraffin-embedded paraganglioma specimens were analyzed by immunohistochemistry, Western blotting, and ELISA.

RESULTS:

Positive immunohistochemical staining for VEGF was observed in five of nine surgical specimens and in six of eight archival specimens (11/17, or 65%). PD-ECGF immunoreactivity was detected in four of five surgical specimens and six of eight archival specimens (10/13, or 77%). The presence of PD-ECGF was confirmed by Western blot assay and ELISA confirmed the presence of VEGF in tumor extract.

CONCLUSIONS:

Both VEGF and PD-ECGF are expressed in paragangliomas and may contribute to the extreme vascularity of these tumors. Key Words. Vascular endothelial growth factor, platelet-derived, endothelial cell growth factor, hypoxia, tumor vasculature.

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