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Fertil Steril. 2000 Jan;73(1):43-50.

Sperm chromatin damage impairs human fertility. The Danish First Pregnancy Planner Study Team.

Author information

1
Section of Toxicology and Biomedical Sciences, ENEA CR Casaccia, Rome, Italy. spanomrc@casaccia.enea.it

Abstract

OBJECTIVE:

To examine the relationship between sperm chromatin defects, evaluated by the flow cytometric (FCM) sperm chromatin structure assay (SCSA), and the probability of a pregnancy in a menstrual cycle (fecundability).

DESIGN:

Follow-up study.

SETTING:

The Section of Toxicology and Biomedical Sciences, ENEA Casaccia, Rome, Italy, and the Department of Occupational Medicine, Aarhus University Hospital, Aarhus, Denmark.

PATIENT(S):

Two hundred fifteen Danish first pregnancy planners with no previous knowledge of their fertility capability.

INTERVENTION(S):

None.

MAIN OUTCOME MEASURE(S):

Semen samples were collected at enrollment to measure semen volume, sperm concentration, motility, and morphology (by microscopy), as well as chromatin susceptibility to in situ, acid-induced partial denaturation by the FCM SCSA. Time to pregnancy was evaluated during a 2-year follow-up period. Demographic, medical, reproductive, occupational, and lifestyle data were collected by questionnaire. Fecundability was correlated with SCSA-derived parameters.

RESULT(S):

Fecundability declines as a function of the percentage of sperm with abnormal chromatin and becomes small when aberrant cells are >40%.

CONCLUSION(S):

Optimal sperm chromatin packaging seems necessary for full expression of the male fertility potential. The SCSA emerged as a predictor of the probability to conceive in this population-based study.

PMID:
10632410
[Indexed for MEDLINE]

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