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Chromosome Res. 1999;7(8):641-7.

A drop-spreading technique to produce cytoplasm-free mitotic preparations from plants with small chromosomes.

Author information

1
The Ecology-Genetics Department, Faculty of Biology-Geology, University Babes-Bolyai, Cluj-Napoca Romania.

Abstract

A preparation technique has been developed for plants with small chromosomes, which produces large numbers of good-quality mitotic preparations. The technique employs a hydrochloric acid treatment to hydrolyse the cytoplasm, facilitating the subsequent removal of cytoplasmic debris. The evaporative force of a methanol-based fixative is exploited to disperse the cytoplasm and to deposit the chromosomes in a single optical plane. This technique permits detailed observations of chromosome morphology and karyotyping. The mitotic preparations are also suitable for the complex analysis associated with in-situ hybridization, as in studies of genome interaction in plant hybrids.

PMID:
10628665
[Indexed for MEDLINE]

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