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Anticancer Res. 1999 Sep-Oct;19(5B):4315-9.

GPI-anchored molecule-like protein (GML) expression in non-small cell lung cancer (NSCLC).

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1
Department of Internal Medicine, Osaka Prefectural Habikino Hospital, Japan.

Abstract

BACKGROUND:

GPI-anchored molecule-like protein (GML) has been identified as a new target molecule of p53. In esophageal cancer cell lines, expression of GML mRNA, which was found to be significantly correlated with in vitro anti-cancer drug sensitivity, was observed only in the presence of wild-type p53.

MATERIALS AND METHODS:

The relationship between expression of GML mRNA and p53 gene status in 24 non-small cell lung cancer (NSCLC) cell lines was examined using RT-PCR and PCR-SSCP followed by direct DNA sequencing.

RESULTS:

Expression of GML mRNA was observed in only 3 of 24 cell lines. In contrast to previous studies showing p53-dependent GML expression, of the 3 cell lines expressing GML mRNA, one had a p53 gene mutation (codon 245: Gly to Cys).

CONCLUSION:

These findings suggest that the expression of GML mRNA is infrequent and regulated in a p53-independent manner in NSCLC.

PMID:
10628393
[Indexed for MEDLINE]
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